APPLIED NANOFLUORESCENCE, LLC — Department of Commerce SBIR Phase II: None

Description

This project will develop a new scientific instrument optimized for the advanced characterization of near-infrared fluorescent nanoparticles that can exist as left- or right-handed structures (enantiomers). Single-walled carbon nanotubes (SWCNTs) are the leading current example of such nanomaterials. Applied NanoFluorescence, LLC (ANF) proposes a novel multi-mode chiroptical spectrometer that can distinguish left and right enantiomers through their different interactions with circularly polarized light. The planned instrument will offer four complementary measurement modes: (1) fluorescence-detected circular dichroism (FDCD) spectra with tunable visible excitation (400-700 nm) and near-infrared emission (900-1600 nm); (2) rapid excitation-emission fluorimetry covering the same visible excitation and near-infrared emission ranges; (3) direct near-infrared circular dichroism absorption spectra; and (4) normal near-infrared absorption spectra. Unlike existing FDCD instruments, mode (1) will offer spectral selection of the emission wavelength, thus allowing structure-specific measurements of CD spectra in unsorted SWCNT samples. Mode (2) will measure full excitation-emission maps with peak signal-to-noise ratios above 100 in less than 2 minutes. All four measurement modes will operate under integrated computer control. The combined results will offer powerful analyses of complex nanoparticle samples and their coatings, guiding research, product development, quality control, and commercial applications.