CLAREMONT BIOSOLUTIONS LLC — Department of Health and Human Services SBIR Phase I: 400

Description

Summary Claremont BioSolutionsLLCCBIOThe ability to sequence and map long stretches of DNA using third generation sequencing technologies is set to transform the field of genomics and allow researchers and clinicians unparalleled insight into the complexities of our genome and diseasesHoweverthe move to these platforms presents new hurdles in long DNA sample preparationMost conventional spin column sample preparation methods shear DNA during purificationAndalthough agarose plug digestion methods can isolate ultra long DNA from samplesthe protocols can be difficulttime consuming and highly variableWhile newer technologies have emerged that allow for more rapid extraction of long DNA these platforms are limited in starting sample type to fresh blood or tissue culture cells and exclude use of most clinically relevant frozen blood and solid tissue samplesAt Claremont BioSolutionsCBIOwe are developing a novel extraction technology that utilizes a proprietary high capacity nucleic acid binding matrixwhich is loosely packed in a low pressure fast flow columnCombined with CBIOandapos s gentle upfront lysis methodthe technology will be compatible with any starting tissue sample typeincluding frozen blood and solid tissueand can be used to isolate ultra long DNA in andltminutesIn Aimwe will demonstrate CBIOandapos s fast flow isolation of high quantityhigh qualityultra long DNAandgtkilobasesfrom fresh and frozen blood and tissue samples and benchmark against existing extraction methodsIn Aimwe will validate isolated ultra long DNA compatibility with two third generation long read sequencing and mapping technologies and demonstrate improved long read length over existing DNA extraction methodsThe future goal of this project will be to develop a platform that will accept a sample and automate all steps of sample lysis and ultra long DNA extraction without user interactionThe proposed system would simplify workflowlower cost and reduce variability Narrative Claremont BioSolutionsLLCCBIOFresh and archived tissue samples offer a wealth of untapped information relevant to cancer and genetic diseasesExtracting high quality ultra long DNA from fresh and frozen blood and solid tissue samples for downstream long read genomic applications is often difficultlabor intensive and highly variableThis project will apply a new technology to allow rapid isolation and reproducible extraction of ultra long DNA for tissue samples for downstream NGS and long read genomic applications