De Novo DNA — Department of Defense SBIR Phase II: SB163-003
De Novo DNA — SBIR Phase II award from Department of Defense.
- Amount
- $1,459,755
- Agency
- Department of Defense · Defense Advanced Research Projects Agency
- Program / Phase
- SBIR · Phase II
- Topic
- SB163-003
- Solicitation
- 16.3
- NAICS
- —
- Place of performance
- PA
- Period
- 2018-09-18 → 2020-10-17
Description
We propose to create transformative technologies to overcome several CRISPR-wide challenges that will broadly enable scalable, multiplexable gene editing & gene regulation with minimal off-target activity. Our technical solutions combine computational design of optimal CRISPR components, genetic circuits, and RNPs together with experimental validation. We will implement several demonstrative applications to exemplify how these technologies fundamentally transform CRISPR from a 1-guide, 1-unique site tool to a many-guide, many-gene platform with many thousands of potential industrial, medical, and agricultural applications.