NANOCELLECT BIOMEDICAL INC — Department of Health and Human Services SBIR Phase II: 400

Description

High throughput single cell sorting and kinetic analysis of secreted particles NanoCellect BiomedicalIncRESEARCH andampRELATED Other Project InformationPROJECT SUMMARY Advances in reagents and analytical tools have improved characterization of cellular phenotypesHowevermost studies are still performed on cell populations that are morphologically and genetically homogeneousyet have important biological heterogeneitywith individual cells having unique phenotypic or `omic profilesThis leads to an average measurement of individual cellsnone of which may actually be averageSingle cell analysis can define the scope of cell to cell variations within a population and provide in depth analysis of singleexceptional cells in contexts such as stem cell differentiation or cancerwhere these cells can give rise to clonal populations that drive the biologyWhile novel tools such as multiparameter flow cytometrymass cytometryand image based cytometry provide in depth analysis of individual cellsthese technologies only provide a single time pointsnapshotand lack downstream molecular analysisThis project will develop for commercialization an integrated cell sortingcell dispensingand single cell positioning device to allow single cells to be tracked and measured over timeIn additionwe will demonstrate a commercial prototype device that allows transfer of individual cells with desired phenotypic characteristics towell plates for downstream analysis in a standard formatThis integrated system will be benchmarked with cells derived from glioblastoma that has been shown to have important exosome biological characteristics High throughput single cell sorting and kinetic analysis of secreted particles NanoCellect BiomedicalIncRESEARCH andampRELATED Other Project InformationPROJECT NARRATIVE The advances proposed here will allow NanoCellect to achieve its missionto advance single cell technology that conforms to standard multi well plate platformsThis will be achieved by integrating a microfluidic cell sorterdispenserwells in a well cell array and cell selection screen to transfer cells of interest towell plates