On Target Laboratories, Inc. — Department of Health and Human Services SBIR Phase I: 102

Description

DESCRIPTION provided by applicant Lung cancer remains the leading cause of cancer related deaths worldwide Surgical removal of the tumor is the best therapeutic option for patients with non small cell lung cancer yet up to of patients still leave the operating room with deposits of cancer left behind Advances in CAT and PET scans have provided surgeons with helpful radiographic imaging information prior to surgery but have limited specificity for nodules less than mm in diameter Subsequent pathological analysis confirms refutes what a surgeon has resected However both of these procedures require additional time and occur outside of the operating room providing little real time support during the surgery The applicant has developed a novel cocktail that combines two ligands targeted specifically to lung tumor tissue tagged with near infrared NIR dyes The fluorescent cocktail containing ligands targeted to both folate receptor and the CCK receptor will essentially identify tumor populations that overexpress either or both of these cell surface receptors Real time visualization not only of the complete tumor margins but also potentially other malignant sites outside the primary tumor area will enable more complete surgical removal of primary and other tumor tissue Moreover fluorescence imaging could aid in the surgical decision to conserve lung function by removing only the diseased tumor tissue rather than lobectomy or pneumonectomy The significance of this tumor targeted fluorescent cocktail is that it will provide real time visualization of tumor tissue during surgery a valuable tool to differentiate tumor from healthy tissue In this Phase I SBIR proposal the applicant will demonstrate the feasibility of co stainin with the cocktail in cell culture and human lung carcinoma tissue samples Studies in mice implanted with tumor cells will demonstrate specificity and sensitivity of the levels of detection n vivo Our commercialization efforts will be aimed at the development of the cocktail for intraoperative use in lung cancer resection surgery The immediate objective of this work is to provide proof of concept for the NIR ligand cocktail both in vitro and in vivo The ultimate goal i to introduce a commercially viable product that will enable more complete tumor removal and a reduction in the recurrence rate of pulmonary cancer and its associated morbidity mortality and expense PUBLIC HEALTH RELEVANCE Lung cancer remains a leading cause of cancer related deaths worldwide Surgical resection is the best therapeutic option for patients with non small lung cell cancer However survival rates are directly affected by incomplete removal of the primary tumor and other malignant sites that cannot be identified The applicant is developing a cocktail of two fluorescent ligands that will be specifically targeted to tumor surface receptors that will visualize and enable image guided resection of lung cancer representing a significant advancement over current surgical limitations in pulmonary resection