BANFIELDBIO INC — Department of Health and Human Services SBIR Phase II: NIAID

Description

DESCRIPTION provided by applicant The urban container breeding mosquitoes Aedes albopictus and Aedes aegypti are vectors for deadly and debilitating diseases such as dengue and Chikungunya There is no known vaccine or treatment for either disease mosquito control is the only effective means of preventing and managing outbreaks But container species are difficult to manage using conventional vector control technologies due to their numerous small cryptic habitats Autodissemination wherein female mosquitoes contaminated with the low risk insect growth regulator pyriproxyfen transport lethal concentrations to additional larval habitats offers the promise of effective economic and environmentally friendly control for these species In Phase I collaborative efforts between SpringStar and the Rutgers University an autodissemination station has been developed and refined for Ae albopictus The station was highly effective in cage and room studies at attracting and contaminating oviposition seeking females and releasing lethal concentrations of pyriproxyfen into subsequent containers visited The Phase I station prototype has high attraction exclusion from oviposition unidirectional design specialized formulations extended activity and is maintenance free biodegradable user friendly and low risk Phase II testing will extend the concept to Ae aegypti assess the effect of field variables on station efficacy assess field deployment on localized mosquito populations in cryptic habitats and modify non essential portions of the station to yield a low cost product Prototype stations will be tested in field and semi field conditions to determine how far the pyriproxyfen can be disseminated how well it reaches cryptic habitats and the impact of competing site abundance size and water quality Cage and room tests will determine effectiveness of the current design against Ae aegypti evaluate alternate oviposition attractants against both Ae albopictus and Ae aegypti and assess the effect of our formulations on adult longevity fecundity and fertility Crucial field trials will center on replicated focal points ofhigh mosquito activity andapos hot spotsandapos Field stored units will be tested over time for structural stabilty efficacy duration and biodegradation The prototype will be modified as necessary to achieve optimal efficacy cost and versatility The final design resulting at the end of this phase will b suitable for large scale production PUBLIC HEALTH RELEVANCE The proposed research will evaluate a simple low to no maintenance device with high potential to control populations of container breeding mosquitoes worldwide thereby lessening a global disease threat and outdoor nuisance This device will provide a novel and highly effective means to target the primary vectors of dengue fever Chikungunya and West Nile Virus and be suitable for cost effective use around the globe with minimal risk to human beings non target animals or the surrounding environment