GUILD ASSOCIATES INC — Department of Health and Human Services SBIR Phase I: DESCRIPTION (provided by applicant): Biological RNAs undergo extensive post-transcription
GUILD ASSOCIATES INC — SBIR Phase I award from Department of Health and Human Services.
- Amount
- $209,008
- Agency
- Department of Health and Human Services · National Institutes of Health
- Program / Phase
- SBIR · Phase I
- Solicitation
- DA15-001
- NAICS
- —
- Place of performance
- OH
- Period
- —
Description
DESCRIPTION (provided by applicant): Biological RNAs undergo extensive post-transcriptional modifications. These modifications are important in promoting the accuracy and efficiency of gene expression in biological systems. To decipher the functions of RNA modifications, robust analytical tools and methods that enable scientists to monitor, manipulate, and transcriptome-wide mapping RNA modifications are highly needed. In this multi-phase SBIR program, Guild Biosciences Inc. (GBS) proposes to address thisissue by: 1) developing a library of affinity antibodies and peptide sequences specific to modified nucleosides by using phase-antibody display libraries and peptide phase-display library, 2) developing a novel immunoassay system- open immunocomplex assaywith the developed antibodies and peptides for rapid and quantitative detection of modified nucleotides in RNA samples, and 3) incorporating the immunoprecipitation capability of the developed affinity reagents with next-generation sequencing platform