MEGASTARTER, LLC — Department of Agriculture SBIR Phase II: Use of high-concentrate diets in cattle production is a common practice in the United Stat

Description

Use of high-concentrate diets in cattle production is a common practice in the United States to maximize production efficiency, enhance meat quality, and minimize meat and milk cost of production. Transition from high-fiber to high-concentrate diets is challenging and put the animals at risk for acidosis. Megastarter LLC is commercializing a liquid Megasphaera elsdenii culture, Lactipro, helping cattle during that transition. Several key factors have limited Lactipro widespread adoption by beef and dairy producers, including the current short shelf life of 14 days and the large dose volume. Phase I research demonstrated cold storage of Lactipro with osmoprotectants and concentration with a filtration system to be feasible. The objective of our Phase II program is to develop a concentrated, lyophilized, second generation Lactipro with extended shelf life and reduced dose size. Phase II research will first implement the filtration system in our production line to concentrate the culture and then investigate optimal freeze drying protocols and storage by testing different combinations of cryoprotectants, freezing treatments, freeze drying cycles, and storage conditions. The most promising combination will then be tested in vivo to confirm efficacy in feedlot cattle.Success of the Phase II research will lead to the commercialization of a second generation Lactipro, which will facilitate adoption by our customers. Extended shelf life and decreased dose size will allow for easier logistics and administration, considerably enhancing end user satisfaction. Additionally, it will permit shipping to non-domestic markets, hence protecting manufacturing trade secrets, creating jobs, and benefiting the American economy.