APPLIED FOOD TECHNOLOGIES, INC — Department of Agriculture SBIR Phase I: 8.5

Description

In Phase I of this project, we will show "proof of concept" that we can develop a rapid method with end-point detection for tuna to differentiate between albacore tuna, and two common adulterants/substitutes for this species-- skipjack tuna and escolar. We propose to streamline the test procedure by eliminating the DNA extraction step (via a direct amplification technology); and replace traditional PCR amplification by conducting isothermal amplification. Once the Phase I objectives are accomplished, in the Phase II, we will optimize these parameters and develop a lateral flow dipstick device for endpoint detection and add additional tuna species and other common tuna substitutes onto the DNA fish stick. Our overarching goal is to deliver a commercialized 3-step lateral flow test that will readily distinguish the eight major, high-valued tuna species in commerce from one another as well as from the commonly substituted species found in commerce. The seafood industry is demanding rapid, user-friendly species identification tests. The entire tuna supply chain (from harvest to consumption) has a critical and immediate need for such a test.