CARDIODX, INC. — Department of Health and Human Services SBIR Phase I: DESCRIPTION (provided by applicant): Current tube-based methods for collecting whole blood
CARDIODX, INC. — SBIR Phase I award from Department of Health and Human Services.
- Amount
- $205,804
- Agency
- Department of Health and Human Services · National Institutes of Health
- Program / Phase
- SBIR · Phase I
- Solicitation
- PAR13-203
- NAICS
- —
- Place of performance
- CA
- Period
- —
Description
DESCRIPTION (provided by applicant): Current tube-based methods for collecting whole blood RNA such as PAXgeneTM have a number of limitations including need for access to phlebotomy, temperature controlled transport, and relatively large volumes of whole blood. No solid-matrix based methods for obtaining RNA from whole blood currently exist. Our goal is the development and implementation of a solid, matrix-based approach for obtaining and stabilizing both RNA and DNA from whole blood, which would address these limitations. In conjunction with investigators at General Electric, we have prototyped an initial platform, term RNA Stabilization Matrix (RSM). In the current proposal we plan to expand upon our initial work in four areas: (1) We plan to develop automated methods using liquid handling robotics for extracting RNA from RSM; such methods will increase throughput as well as decrease variability associated with manual processing. (2) We plan to develop methods allowing for whole transcriptome analysi