RESODYN CORPORATION — Department of Health and Human Services SBIR Phase I: 400

Description

This Small Business Innovation Research Phase I project will develop a prototype and then evaluate a novel device that utilizes low frequency sound energy to enhance cell culture productivityThe acoustic micromixing generated by the proposed device is compatible with cells and possesses the capability to substantially enhance the culture efficiency of cells cultured in typical culture vesselsThis approach is not ultrasonic which has very limited penetration range and can damage cellsMaintaining consistent oxygen and nutrient profiles is extremely important in order to deliver highly efficientrepeatableand scalable protocols for the large scale production of cells for stem cell and regenerative medicine applications and for robust process development of mammalian cell culture derived pharmaceutical productsThe approach proposed here is to develop a single incubator like acoustic platform on which multiple cell culture vessels can be mounted to deliver similar benefits as those derived from bioreactors but in a simpler and more versatile deviceThe enabling advantage of low frequency sound energy is the ability to non invasively generate low shearmicro scale mixing inside closed culture vessels that can enhance the movement of liquidmoleculesand oxygen without pumpsbioreactorsor other complex componentsThis will result in a substantial increase in productivity for the scale up of stem cell and regenerative medicine productsPreliminary data is very promising and strongly supports the feasibility of the proposed technologyThe commercial applications of the device would be broad for enhancing the growth of stem cells and other types of cellsthus reducing labor requirements and reducing the production cost of cell based medical therapeutics This Small Business Innovation Research Phase I project will develop a prototype acoustic culture enhancement device that utilizes low frequency acoustic sound waves to significantly improve the efficiency of cell culture for cellular therapy and regenerative medicine applicationsIn order to effectively scale up and reduce the costs of bringing cellular therapies to the clinicmore time efficient and cost effective means of producing the large numbers of cells required to facilitate cellular and gene therapy are neededDevelopment of the proposed device will meet this need that is currently not addressed by current technologies